期刊
ACS CATALYSIS
卷 8, 期 3, 页码 2611-2614出版社
AMER CHEMICAL SOC
DOI: 10.1021/acscatal.7b04369
关键词
biohybrid catalysis; whole cell catalysis; cell surface display; protein engineering metalloenzymes
资金
- JSPS KAKENHI [JP15H05804]
Whole cell catalysis is, in many cases, a prerequisite for the cost-effective production of chemicals by biotechnological means. Synthetic metal catalysts for bioorthogonal reactions can be inactivated within cells due to abundant thiol derivatives. Here, a cell surface display-based whole cell biohybrid catalyst system (termed ArMt bugs) is reported as a generally applicable platform to unify cost-effective whole cell catalysis with biohybrid catalysis. An inactivated esterase autotransporter is employed to display the nitrobindin protein scaffold with a Rh catalyst on the E. coli surface. Stereoselective polymerization of phenylacetylene yielded a high turnover number (TON) (39 X 10(6) cell(-1)) for the ArMt bugs conversion platform.
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