期刊
NATURE COMMUNICATIONS
卷 9, 期 -, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-018-03084-4
关键词
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资金
- National Natural Science Foundation of China [21750001, 21621004, 21676192]
- Ministry of Science and Technology of China (973 Program) [2014CB745100]
- International S&T Cooperation Program of China [2015DFA00960]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM007308, T32GM066704] Funding Source: NIH RePORTER
- Direct For Biological Sciences [1616111] Funding Source: National Science Foundation
Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast cells incorporating the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system provide a platform for generating genotype diversity. Here we construct a genetic AND gate to enable precise control of the SCRaMbLE method to generate synthetic haploid and diploid yeast with desired phenotypes. The yield of carotenoids is increased to 1.5-fold by SCRaMbLEing haploid strains and we determine that the deletion of YEL013W is responsible for the increase. Based on the SCRaMbLEing in diploid strains, we develop a strategy called Multiplex SCRaMbLE Iterative Cycling (MuSIC) to increase the production of carotenoids up to 38.8-fold through 5 iterative cycles of SCRaMbLE. This strategy is potentially a powerful tool for increasing the production of bio-based chemicals and for mining deep knowledge.
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