CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPRFRT circumvents this design constraint to provide a broad platform for fast, scarless, off-theshelf genome engineering.