期刊
NATURE COMMUNICATIONS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-03902-9
关键词
-
资金
- European Research Council [261227]
- Wellcome Trust [110164/Z/15/Z]
- UK BBSRC [BB/H01795X/1, BB/J00054X/1]
- US National Science Foundation grant [1309306]
- EPA Cephalosporin Junior Research Fellowship at Linacre College, Oxford
- Interdisciplinary Center for Clinical Research (IZKF) at the University Hospital of the University of Erlangen-Nuremberg
- Instrumentarium Science Foundation
- Finnish Cultural Foundation
- Alfred Kordelin Foundation
- Russian Foundation for Basic Research grant [17-54-150009]
- BBSRC [BB/H01795X/1, BB/J00054X/1] Funding Source: UKRI
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1309306] Funding Source: National Science Foundation
Transcription in bacteria is controlled by multiple molecular mechanisms that precisely regulate gene expression. It has been recently shown that initial RNA synthesis by the bacterial RNA polymerase (RNAP) is interrupted by pauses; however, the pausing determinants and the relationship of pausing with productive and abortive RNA synthesis remain poorly understood. Using single-molecule FRET and biochemical analysis, here we show that the pause encountered by RNAP after the synthesis of a 6-nt RNA (ITC6) renders the promoter escape strongly dependent on the NTP concentration. Mechanistically, the paused ITC6 acts as a checkpoint that directs RNAP to one of three competing pathways: productive transcription, abortive RNA release, or a new unscrunching/scrunching pathway. The cyclic unscrunching/scrunching of the promoter generates a long-lived, RNA-bound paused state; the abortive RNA release and DNA unscrunching are thus not as tightly linked as previously thought. Finally, our new model couples the pausing with the abortive and productive outcomes of initial transcription.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据