期刊
NATURE COMMUNICATIONS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-04402-6
关键词
-
资金
- Swiss National Science Foundation (SNSF) [PP00P3_139093]
- SNSF R'Equip [150838]
- Marie Curie Career Integration Grant from the European Commission [CIG PCIG14-GA-2013-631984]
- Canton of Zurich
- UZH Foundation for Research in Science and the Humanities
- Swiss Heart Foundation
- Helmholtz Young Investigator Program [VH-NG-736]
- Deutsche Forschungsgemeinschaft (DFG) [PA2619/1-1]
- Marie Curie Career Integration Grant from the European Commission (WNT/CALCIUM IN) [HEART-322189]
- Company of Biologists and EuFishBioMed
- Swiss National Science Foundation (SNF) [PP00P3_139093] Funding Source: Swiss National Science Foundation (SNF)
The vertebrate heart develops from several progenitor lineages. After early-differentiating first heart field (FHF) progenitors form the linear heart tube, late-differentiating second heart field (SHF) progenitors extend the atrium and ventricle, and form inflow and outflow tracts (IFT/OFT). However, the position and migration of late-differentiating progenitors during heart formation remains unclear. Here, we track zebrafish heart development using transgenics based on the cardiopharyngeal gene tbx1. Live imaging uncovers a tbx1 reporter-expressing cell sheath that continuously disseminates from the lateral plate mesoderm towards the forming heart tube. High-speed imaging and optogenetic lineage tracing corroborates that the zebrafish ventricle forms through continuous addition from the undifferentiated progenitor sheath followed by late-phase accrual of the bulbus arteriosus (BA). FGF inhibition during sheath migration reduces ventricle size and abolishes BA formation, refining the window of FGF action during OFT formation. Our findings consolidate previous end-point analyses and establish zebrafish ventricle formation as a continuous process.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据