期刊
ONCOLOGY LETTERS
卷 16, 期 2, 页码 1967-1974出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2018.8854
关键词
breast cancer cell; apoptosis; acetylation; synergistic effect; fluorouracil
类别
资金
- National Natural Science Foundation of China [81201550, 81560452, 81672866]
- Zhujiang Star 2015 of Science and Technology Program of Guangzhou, China [201506010037]
- Natural Science Foundation of Jiangxi Province [20161BAB205192, 20171ACB21073]
- Excellent Youth Foundation of Jiangxi Scientific Committee [20162BCB23001]
- Science and Research Fund of Jiangxi Health and Family Planning Commission [20164002]
- Foundation of Nanchang Science and Technology Bureau [2016 ZSCX 009]
AR-42 is a member of a novelly discovered class of phenylbutyrate-derived histone deacetylase inhibitors, and has a number of antitumor effects in a variety of tumor types; however, the role of AR-42 and its possible mechanisms have not been reported in the treatment of breast cancer. The aim of the present study was to investigate the antitumor effects of AR-42 and its associated mechanisms in breast cancer. MTT assays and colony formation assays were conducted to measure the proliferation of MCF-7 cells, and flow cytometry was used to analyze cell apoptosis. The results revealed that AR-42 induced cell apoptosis and suppressed cell growth in a dose- and time-dependent manner. Mechanistically, AR-42 treatment increased the acetylation of the p53 protein and prolonged the half-life of the p53 protein; furthermore, AR-42 treatment upregulated p21 and PUMA expression. Notably, AR-42 had a synergistic effect on MCF-7 cells in combination with fluorouracil, which is one of the most commonly used chemotherapeutic agents. In conclusion, the results indicated that AR-42 inhibits breast cancer cell proliferation and induces apoptosis, indicating that AR-42 is a potential therapeutic agent.
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