期刊
EPIGENOMICS
卷 10, 期 2, 页码 199-218出版社
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2017-0071
关键词
DNA methylation; evolution; new gene; retrogenes; two-step strategy
资金
- National Natural Science Foundation of China (NSFC) [31601859, 31472076]
- Research Project of Chinese Ministry of Education [113048A]
- National Basic Research Priorities Program of China (973 Program) [2013CB835205]
- Science Fund for Distinguished Young Scholars of Hubei Province of China [2014CFA024]
Aim: This study aims to couple DNA methylation changes and evolution of retrogenes. Materials & methods: A new two-step strategy was developed to screen retrogenes. Further, reduced representation bisulfite sequencing and RNA-seq data of eight tissues were used to analyze retrogenes. Results: A total of 964 retrocopies were identified and new retrocopies were available for the synthesis of glycans and lipids corresponding to pig phenotypic traits. Retrogenes were consistently hypermethylated. Hypomethylation of parental genes presented more susceptibility to retroposition. Promoter DNA methylation of retrogenes was negatively correlated with evolutionary time and played important roles in regulating retrogene tissue-specific expression pattern. Conclusion: A two-step procedure is effective and necessary for identifying retrogenes. DNA methylation drives origination, survival, evolution and expression of retrogenes.
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