Grail is involved in adipocyte differentiation and diet-induced obesity

Grail is a crucial regulator of various biological processes, including the development of T cell anergy, antiviral innate immune response, and cancer However, the role of Grail in adipogenesis and obesity remains unclear Here, we demonstrated that Grail knockdown in vitro leads to a decrease in PPARy expression, resulting in adipogenesis inhibition However, Grail overexpression induced the same effects Grail was shown to interact with PPARy, targeting it for degradation and modulating its adipogenic activity PPARy expression was shown to be considerably reduced in Grail knockout (KO) mice fed normal diet or high fat diet (HFD) The administration of both normal diet or HFD to Grail KO mice led to lower adipose mass and body weight than those in the wild type mice HFD fed Grail KO mice had improved glucose and insulin tolerance Taken together, our results indicate that Grail plays a pivotal role in adipogenesis and diet induced obesity by regulating PPARy activity.