Ginsenosides Rg5 and Rkl, the skin-whitening agents in black ginseng

Ginseng (Pcunix ginseng Meyer) is widely used to treat various chronic disorders. Black ginseng is obtained by repeated steaming and drying of raw ginseng, which turn it black and convert the ginsenoside compounds present into more potent bioactive ingredients. The effects of a black ginseng extract on melanin production and skin whitening were evaluated in vitro as well as in vivo using human skin and zebrafish embryos. Tyrosinase activity decreased when steamed and dried ginseng was used. A pronounced skin-whitening effect was observed in our clinical study, and inhibition of melanin activity and tyrosinase levels was confirmed in zebrafish embryos. Two ginsenosides specific to black ginseng activate the MEK-ERK signaling pathway and are the main factors responsible for skin whitening. The skin-whitening effects of black ginseng are associated with the formation of two ginsenosides, indicating that black ginseng can be used as a novel skin-whitening agent in cosmetic products.