期刊
BMC NEPHROLOGY
卷 19, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12882-018-0968-4
关键词
Podocytes; Apoptosis; Angiotensin II; PKC-delta; p38 MAPK; NHE1
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/19569-3, 17/02020-0, 13/23087-4, 14/19154-0, 14/17251-9, 11/14022-0]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/23087-4, 13/19569-3, 14/19154-0, 14/17251-9, 11/14022-0, 17/02020-0] Funding Source: FAPESP
Background: Angiotensin II (Ang II) contributes to the progression of renal diseases associated with proteinuria and glomerulosclerosis mainly by inducing podocyte apoptosis. In the present study, we investigated whether the chronic effects of Ang II via AT1 receptor (AT1R) would result in endoplasmic reticulum (ER) stress/PKC-delta/p38 MAPK stimulation, and consequently podocyte apoptosis. Methods: Wistar rats were treated with Ang II (200 ng.kg(-1).min(-1), 42 days) and or losartan (10 mg.kg(-1).day(-1), 14 days). Immortalized mouse podocyte were treated with 1 mu M Ang II and/or losartan (1 mu M) or SB203580 (0.1 mu M) (AT1 receptor antagonist and p38 MAPK inhibitor) for 24 h. Kidney sections and cultured podocytes were used to evaluate protein expression by immunofluorescence and immunoblotting. Apoptosis was evaluated by flow cytometry and intracellular pH (pHi) was analyzed using microscopy combined with the fluorescent probe BCECF/AM. Results: Compared with controls, Ang II via AT1R increased chaperone GRP 78/Bip protein expression in rat glomeruli (p < 0.001) as well as in podocyte culture (p < 0.01); increased phosphorylated eIf2-alpha (p < 0.05), PKC-delta (p < 0.01) and p38 MAPK (p < 0.001) protein expression. Furthermore, Ang II induced p38 MAPK-mediated late apoptosis and increased the Bax/Bcl-2 ratio (p < 0.001). Simultaneously, Ang II via AT1R induced p38 MAPK-NHE1-mediated increase of pHi recovery rate after acid loading. Conclusion: Together, our results indicate that Ang II-induced podocyte apoptosis is associated with AT1R/ER stress/PKC-delta/p38 MAPK axis and enhanced NHE1-mediated pHi recovery rate.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据