期刊
CHEMICAL SCIENCE
卷 9, 期 7, 页码 1774-1781出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7sc05246d
关键词
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资金
- National Natural Science Foundation of China [21575138, 21775108]
- China International Science and Technology Cooperation Based of Food Nutrition/Safety and Medicinal Chemistry
- Tianjin Municipal Science and Technology Commission [16PTSYJC00130]
- International Science and Technology Cooperation Program of China [2014DFR30350]
The integration of multiple DNA logic gates on a universal platform to implement advance logic functions is a critical challenge for DNA computing. Herein, a straightforward and powerful strategy in which a guanine-rich DNA sequence lighting up a silver nanocluster and fluorophore was developed to construct a library of logic gates on a simple DNA-templated silver nanoclusters (DNA-AgNCs) platform. This library included basic logic gates, YES, AND, OR, INHIBIT, and XOR, which were further integrated into complex logic circuits to implement diverse advanced arithmetic/non-arithmetic functions including half-adder, half-subtractor, multiplexer, and demultiplexer. Under UV irradiation, all the logic functions could be instantly visualized, confirming an excellent repeatability. The logic operations were entirely based on DNA hybridization in an enzyme-free and label-free condition, avoiding waste accumulation and reducing cost consumption. Interestingly, a DNA-AgNCs-based multiplexer was, for the first time, used as an intelligent biosensor to identify pathogenic genes, E. coli and S. aureus genes, with a high sensitivity. The investigation provides a prototype for the wireless integration of multiple devices on even the simplest single-strand DNA platform to perform diverse complex functions in a straightforward and cost-effective way.
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