期刊
CHEMICAL SCIENCE
卷 9, 期 24, 页码 5372-5382出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8sc01611a
关键词
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资金
- National Natural Science Foundation of China [21375027, 21335002, 21427806]
- Shanghai Pujiang Program [17PJD001]
- Natural Science Foundation of Shanghai [12ZR1401700]
Early diagnosis and metastasis monitoring for pancreatic cancer are extremely difficult due to a lack of sensitive liquid biopsy methods and reliable biomarkers. Herein, we developed easy-to-prepare and effective polydopamine-modified immunocapture substrates and an ultrathin polydopamine-encapsulated antibody-reporter-Ag(shell)-Au(core) multilayer (PEARL) Surface-Enhanced Raman Scattering (SERS) nano-tag with a quantitative signal of the Raman reporter at 1072 cm(-1), which achieved ultrasensitive and specific detection of pancreatic cancer-derived exosomes with a detection limit of only one exosome in 2 L of sample solution (approximately 9 x 10(-19) mol L-1). Furthermore, by analyzing a 2 L clinical serum sample, the migration inhibitory factor (MIF) antibody-based SERS immunoassay could not only discriminate pancreatic cancer patients (n = 71) from healthy individuals (n = 32), but also distinguish metastasized tumors from metastasis-free tumors, and Tumor Node Metastasis (TNM) P1-2 stages from the P3 stage (the discriminatory sensitivity was 95.7%). Thus, this novel immunoassay provides a powerful tool for the early diagnosis, classification and metastasis monitoring of pancreatic cancer patients.
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