期刊
CHEMICAL SCIENCE
卷 9, 期 16, 页码 3906-3910出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7sc05325h
关键词
-
资金
- U.S. National Institutes of Health [GM124316]
While it has been well recognized that affordable and pocket-size devices play a major role in environmental monitoring, food safety and medical diagnostics, it often takes a tremendous amount of resources to develop such devices. Devices that have been developed are often dedicated devices that can detect only one or a few targets. To overcome these limitations, we herein report a novel target-responsive smart thermometer for translating molecular detection into a temperature test. The sensor system consists of a functional DNA-phospholipase A(2) (PLA(2)) enzyme conjugate, a liposome-encapsulated NIR dye, and a thermometer interfaced with a NIR-laser device. The sensing principle is based on the target-induced release of PLA(2) from the DNA-enzyme conjugate, which catalyzes the hydrolysis of liposome to release the NIR dye inside the liposome. Upon NIR-laser irradiation, the released dye can convert excitation energy into heat, producing a temperature increase in solution, which is detectable using a thermometer. Considering the low cost and facile incorporation of the system with suitable functional DNAs to recognize many targets, the system demonstrated here makes the thermometer an affordable and pocket-size meter for the detection and quantification of a wide range of targets
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