期刊
FOOD AND ENVIRONMENTAL VIROLOGY
卷 10, 期 3, 页码 297-304出版社
SPRINGER
DOI: 10.1007/s12560-018-9343-7
关键词
Pepper mild mottle virus; Wastewater; Enteric virus; Persistence; Effluent-dominated river; Quantitative PCR
This study was designed to determine the quantitative polymerase chain reaction (qPCR) signal persistence of viruses in three effluent-dominated streams. Samples were collected from the effluent outfall of three wastewater treatment plants in the Western United States and downstream at different locations. All samples were tested for the presence of pepper mild mottle virus (PMMoV), adenoviruses, norovirus GI and GII, Aichi virus, and enteroviruses using qPCR. PMMoV was detected most frequently in 54/57 (94.7%) samples, followed by adenoviruses which was detected in 21/57 (36.8%) samples. PMMoV was detected at all locations downstream and up to 32 km from the discharge point. This study demonstrated that the detection signal of PMMoV was able to persist in wastewater discharges to a greater degree than human enteric viruses in effluent-dominated rivers.
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