期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 34, 期 6, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-018-2458-z
关键词
Biotransformation; Coculture; Glycosylation; Polyphenols; Polydatin; Resveratroloside
资金
- Vietnam National Foundation for Science and Technology Development (NAFOSTED) [106-NN.02-2014.25]
- NNF Center for Biosustainability [iLoop] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
In bio-based fermentation, the overall bioprocess efficiency is significantly affected by the metabolic burden associated with the expression of complete biosynthetic pathway as well as precursor and cofactor generating enzymes into a single microbial cell. To attenuate such burden by compartmentalizing the enzyme expression, recently synthetic biologists have used coculture or poly-culture techniques for biomolecules synthesis. In this paper, coculture system of two metabolically engineered Escherichia coli populations were employed which comprises upstream module expressing two enzymes converting para-coumaric acid into resveratrol and the downstream module expressing glucosyltransferase to convert the resveratrol into its glucosidated forms; polydatin and resveratroloside. Upon optimization of the initial inoculum ratio of two E. coli populations, 92 mg resveratrol glucosides/L (236 A mu M) was produced i.e. achieving 84% bioconversion from 280 A mu M of p-coumaric acid in 60 h by 3 L fed batch fermentor. This is the report of applying coculture system to produce resveratrol glucosides by expressing the aglycone formation pathway and sugar dependent pathway into two different cells.
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