Effect of aromatic repolymerization of humic acid-like fraction on digestate phytotoxicity reduction during high-solid anaerobic digestion for stabilization treatment of sewage sludge

Humification plays an important role in the sewage sludge stabilization treatment and probably influences the end-product ecotoxicity, which are not fully understood for high-solid anaerobic digestion (AD) process. This study monitored the aromaticity degree of humic acid-like fraction (HA) and detected digestate phytotoxicity during a 48-d high-solid AD. Significant repolymerization of aromatic structures in HA. Electrical conductivity (EC) of digestate changed negatively with the aromaticity degree in HA, and the digestate with high EC had low seed germination index (SGI). A positive correlation between SGI and the aromaticity degree of HA was found. Aromatic repolymerization of HA supplies more aromatic electron-rich sites to form neo-humic polymers and to determine the distribution of organic and/or inorganic phytotoxic compounds; thus, aromatic repolymerization of HA may control the digestate phytotoxicity by reducing excessive salinity. The results showed the effect of HA repolymerization on digestate stabilization and phytotoxicity reduction during the high-solid AD process. The inadequate phytotoxicity reduction efficiency (SGI <= 17%) may be ascribed to unaccomplished HA repolymerization, creating a demand for humification intensification studies. (C) 2018 Elsevier Ltd. All rights reserved.