期刊
WATER RESEARCH
卷 139, 期 -, 页码 263-271出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2018.04.019
关键词
Vegetable waste; Thermophilic digestion; Process monitoring; Instability diagnosis; Syntrophic acetate oxidization
资金
- National Natural Science Foundation of China [21476222, 51478448]
- Youth Innovation Promotion Association of Chinese Academy of Sciences [2017423]
- Program of Strategic Resource Service Network of Chinese Academy of Sciences [ZSYS-009]
- Science and Technology Service Network Initiative (STS) of Chinese Academy of Sciences
- Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences
- Chengdu Science and Technology Huimin Project [2016-HM02-00092-SF]
Effective process monitoring and instability diagnosis are important for stable anaerobic digestion (AD) of vegetable waste (VW). In order to evaluate the performance of thermophilic digestion of VW, to make early diagnosis for instability after organic overload, and to reveal the dynamics of microbial community under different running states, thermophilic AD of VW was carried out under improved organic loading rates (OLR) of 0.5-2.5 g volatile solid (VS)/(L . d) in this study. Gaseous parameters including volumetric methane production rate (VMPR), CH4, CO2, and H-2 concentrations, and liquid parameters including pH, oxidation reduction potential, volatile fatty acid (VFA), and total alkalinity (TA), bicarbonate alkalinity (BA), intermediate alkalinity (IA), and ammonia, were monitored. The coupling parameters, such as the CH4/CO2, VFA/BA, and BA/TA ratios were also used to evaluate stability. The dynamics of syntrophic acetate-oxidizing bacteria (SAOB), acetoclastic methanogens (AM), and hydrogenotrophic methanogens (HM) were analyzed by high-throughput sequencing. The main methanogenic bacteria were HM (Methanothermobacter) during the start-up period of OLR 0.5 gVS/(L . d), while they were AM (Methanosarcina) during the stable period of OLR of 1.0 gVS/(L . d). The VMPR of stable period was about 0.29 L/(L . d) with total VFA concentration below 100 mg/L, CH4/CO2 > 1.3, and BA/TA>0.9. The first instability due to the accumulation of VFA and self-recovery due to syntrophic acetate oxidation occurred at an OLR of 1.5 g VS/(L . d). The syntrophic acetate-oxidizing bacteria probably belong to genus S1 (family Thermotogaceae). The digestion failed at an OLR of 2.0 g VS/(L . d). H-2 was only detected during collapsed period instead of instable period. The total ammonia nitrogen loss and bicarbonate alkalinity (BA) reduction were the primary causes for the instability of AD of VW without effluent recirculation. Compared with single parameters, the CH4/CO2 and BA/total alkalinity (TA) ratios are recommended as early warning indicators for engineering applications of thermophilic AD of VW. (C) 2018 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据