期刊
VIROLOGY
卷 521, 期 -, 页码 175-180出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2018.06.007
关键词
Mosquito; Virus; Cell culture; Insect-specific viruses
类别
资金
- Centers for Disease Control and Prevention [U50/CCU116806-01, U01/CK000509-01]
- US Department of Agriculture Hatch Funds and Multistate Research Project [CONH00773, NE1443]
- National Institute of Health, National Institute of Allergy and Infectious Diseases [AI067380, AI099042]
- NIH/NCATS [UL1 TR001082]
Mosquito cell lines have been used extensively in research to isolate and propagate arthropod-borne viruses and understand virus-vector interactions. Despite their utility as an in vitro tool, these cell lines are poorly defined and may harbor insect-specific viruses. Accordingly, we screened four commonly-used mosquito cell lines, C6/36 and U4.4 cells from Aedes albopictus, Aag2 cells from Aedes aegypti, and Hsu cells from Culex quinquefasciatus, for the presence of adventitious (i.e. exogenous) viruses. All four cell lines stained positive for double-stranded RNA, indicative of RNA virus replication. We subsequently identified viruses infecting Aag2, U4.4 and Hsu cell lines using untargeted next-generation sequencing, but not C6/36 cells. PCR confirmation revealed that these sequences stem from active viral replication and/or integration into the cellular genome. Our results show that these commonly-used mosquito cell lines are persistently-infected with several viruses. This finding may be critical to interpreting data generated in these systems.
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