期刊
VIROLOGY
卷 513, 期 -, 页码 153-159出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2017.10.014
关键词
Glycine max (L.) Merr.; Soybean mosaic virus; Resistance gene; Rsv3
类别
资金
- Next Generation BioGreen 21 Program [PJ01101401]
- Rural Development Administration
- Vegetable Breeding Research Center through Agriculture, the Food and Rural Affairs Research Center Support Program from the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [710001-3]
- Brain Korea 21 Plus Project
Soybean mosaic virus (SMV), a member of the genus Potyvirus, significantly reduces soybean production worldwide. Rsv3, which confers strain-specific resistance to SMV, was previously mapped between the markers A519F/R and M3Satt in chromosome 14 of the soybean [Glycine max (L.) Mem] genotype L29. Analysis of the soybean genome database revealed that five different NBS-LRR sequences exist between the flanking markers. Among these candidate Rsv3 genes, the full-length cDNA of the Glyma.14g204700 was successfully cloned from L29. Over-expression of Glyma.14g204700 in leaves inoculated with SMV inhibited viral infection in a soybean genotype lacking Rsv3. In addition, the transient silencing of the candidate gene caused a high accumulation of an avirulent strain in L29 carrying Rsv3. Our results therefore provide additional line of evidence to support that Glyma.14g204700 is likely Rsv3 gene that confers strain-specific resistance to SMV.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据