期刊
ULTRASONICS SONOCHEMISTRY
卷 48, 期 -, 页码 275-280出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2018.05.033
关键词
Catechol; Polymerization; Laccase; Ultrasonic bath; High-pressure homogenizer
资金
- Chinese Government Scholarship under China Scholarship Council [201606790036]
- Chinese Foundation Key projects of governmental cooperation in international scientific and technological innovation [2016 YFE0115700]
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469/2013]
- COMPETE 2020 [POCI-01-0145-FEDER-006684]
- BioTecNorte operation - European Regional Development Fund under the scope of Norte2020 Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004]
- European Union through the European Regional Development Fund (ERDF) under the Competitiveness Operational Program [COP-A1.1.4-E-2015 nr.30/01.09.2016]
- Project Search-ON2: Revitalization of HPC infrastructure of UMinho - North Portugal Regional Operational Programme, under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) [NORTE-07-0162-FEDER-000086, ON.2]
- FCT-Fundacao para a Ciencia e a Tecnologia [SFRH/BD/121673/2016]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/121673/2016] Funding Source: FCT
The laccase polymerization of catechol was performed using different reactors namely a water bath (WB), an ultrasonic bath (US) and a high-pressure homogenizer (HPH). The total content of free OH and the MALDI-TOF spectra of polymers obtained demonstrated that reactions are favored in the presence of high-energy environments. Higher conversion yields and polymerization degrees (DP) were obtained after polymerization using US or HPH. Molecular dynamic simulation studies supported these findings by revealing a more open enzyme active site upon environments with high molecular agitation. The higher mass transport generated by US and HPH is the main feature responsible for a higher substrate accessibility to the enzyme which contributed to produce longer polymers.
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