4.2 Article

Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase

期刊

TURKISH JOURNAL OF CHEMISTRY
卷 42, 期 2, 页码 346-354

出版社

Tubitak Scientific & Technological Research Council Turkey
DOI: 10.3906/kim-1708-68

关键词

Molecularly imprinted polymers; biomimetic sensors; tyrosinase; electropolymerization; scopoletin; ophenylenediamine

资金

  1. Deutsche Forschungsgemeinschaft (DFG) [EXC 314]
  2. Turkish-German University Scientific Research Projects Commission [2016BF0011]

向作者/读者索取更多资源

For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R-2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.

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