期刊
TRENDS IN BIOCHEMICAL SCIENCES
卷 43, 期 6, 页码 436-451出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibs.2018.02.012
关键词
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资金
- French Government under the program 'Investissements d'Avenir' [ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001-02PSL*]
- European Research Council (ERC Advanced Grant) [693021]
- European Commission (Marie Sklodowska-Curie fellowship) [701467]
- Deutsche Forschungsgemeinschaft (DFG) [KL 2935/1-1]
- Marie Curie Actions (MSCA) [701467] Funding Source: Marie Curie Actions (MSCA)
- European Research Council (ERC) [693021] Funding Source: European Research Council (ERC)
Approaches to remotely control and monitor ion channel operation with light are expanding rapidly in the biophysics and neuroscience fields. A recent development directly introduces light sensitivity into proteins by utilizing photosensitive unnatural amino acids (UAAs) incorporated using the genetic code expansion technique. The introduction of UAAs results in unique molecular level control and, when combined with the maximal spatiotemporal resolution and poor invasiveness of light, enables direct manipulation and interrogation of ion channel functionality. Here, we review the diverse applications of light-sensitive UAAs in two superfamilies of ion channels (voltage- and ligand-gated ion channels; VGICs and LGICs) and summarize existing UAA tools, their mode of action, potential, caveats, and technical considerations to their use in illuminating ion channel structure and function.
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