期刊
TRANSFUSION MEDICINE AND HEMOTHERAPY
卷 45, 期 1, 页码 33-41出版社
KARGER
DOI: 10.1159/000475845
关键词
CD45 antigen; Leukocyte; Platelet activation; Platelet concentrates; Reactive oxygen species
资金
- Iranian blood transfusion organization [1393-01-33-1781]
- High Institute for Research and Education in Transfusion Medicine in Iran
Background: Platelet stimulation with agonists is accompanied by the generation of reactive oxygen species (ROS) which promotes further platelet activation and aggregation. Considering different cell populations in platelet concentrates (PCs), this study investigates the correlation of ROS generation with the expression and release of platelet activation markers during storage. Methods: Samples obtained from 6 PCs were subjected to flow cytometry and ELISA to evaluate the expression and shedding of platelet P-selectin or CD40L during storage. Intracellular ROS were detected in either CD45- or CD45+ population by flow cytometry using dihydrorhodamine 123, while ROS production was analyzed in both P-selectin+ or P-selectin-and CD40L+ or CD40L-populations. To further evaluate the correlation between ROS generation and release function, TRAP-stimulated platelets were also subjected to flow cytometry analysis. Results: ROS detected in the CD45-population (leukocyte-free platelets) was significantly increased by fMLP and PMA. P-selectin-or CD40L-platelet did not show significant amount of ROS. Total ROS generation was significantly increased during platelet storage (day 0 vs. day 5; p = 0.0002) while this increasing pattern was directly correlated with the expression of P-selectin (r = 0.72; p = 0.0001) and CD40L (r = 0.69; p = 0.0001). ROS generations were significantly correlated with ectodomain shedding of these pro-inflammatory molecules. Conclusion: Our data confirmed increasing levels of intracellular ROS generation in both platelets (CD45-) and platelet-leukocyte aggregates (CD45+) during PC storage. The amount of detected ROS is directly correlated with platelet activation and release in each population while platelet-leukocyte aggregates generate higher levels of ROS than single platelets. (c) 2018 S. Karger GmbH, Freiburg
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