期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 65, 期 6, 页码 1770-1774出版社
WILEY
DOI: 10.1111/tbed.12950
关键词
ELISA; emerging zoonoses; Europe; hepatitis E virus; RT-qPCR; wildlife
资金
- Ministerstvo Zdravotnictvi Ceske Republiky [17-31921A]
To determine whether hepatitis E virus (HEV) was distributed in the population of wild boars in South Moravia between 1990 and 2008, a total of 366 samples of archived sera from wild boars were investigated using serological (commercial ELISA) and molecular (RT-qPCR) methods. A total of 31 (8.5%) wild boars were seropositive, and from two of them, RNA sequences were recovered by nested RT-PCR. The presented results, with one of the oldest animal ' s HEV-positive serum (collected in 1990), suggest that wild boars may be a reservoir of HEV in the Czech Republic and that this virus has been circulating in studied areas for more than 20 years.
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