期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 102, 期 -, 页码 332-342出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2018.03.011
关键词
Enzyme immobilization; Immobilization methods; Easy separated support materials; MNPs; Membranes; Capillary columns
资金
- National Natural Science Foundation of China [21775153, 21575150]
Compared with free enzymes, immobilized enzymes are more robust and resistant to environmental changes. In addition, with enhanced stability, immobilized enzymes can be separated from the reaction mixture and used for repeated cycles. These advantages prompt their applications in various fields. This review outlines the existing methods and easy separated support materials for enzymes immobilization. After a brief introduction on the immobilized enzyme, the immobilization methods of adsorption, entrapment, covalent attachment and cross-linking are discussed. The emphasis is given on the easy separated support materials of magnetic nanoparticles (MNPs), membranes and capillary columns. An outlook on the immobilized enzyme is given at last. (C) 2018 Elsevier B.V. All rights reserved.
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