期刊
TOXICOLOGY LETTERS
卷 285, 期 -, 页码 156-167出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2018.01.004
关键词
Silver nanoparticles; SH-SY5Y cells; Endoplasmic reticulum stress; PTEN; Mitochondria-associated membranes; Inositol-3-phosphate receptor
类别
资金
- National Science and Technology Support Program [2012BAI22B01]
- Natural Science Foundation of Liaoning Province of China [201602704]
Silver nanoparticles (AgNPs) have many medical and commercial applications, but their effects on human health are poorly understood. The aim of this study was to assess the effect of AgNPs on the human neuroblastoma cell line SH-SY5Y and to explore their potential mechanisms of action. We found that AgNPs decreased SH-SY5Y cell viability in a dose-and time-dependent manner. Exposure to AgNPs activated endoplasmic reticulum (ER) stress, as reflected by upregulated expression of glucose-regulated protein 78 (GRP78), phosphorylated PKR-like endoplasmic reticulum kinase (p-PERK), phosphorylated eukaryotic translation initiation factor 2a (p-eIF2a), C/ EBP homology protein (CHOP), spliced X-box binding protein-1 (XBP1), and phosphorylated inositol-requiring enzyme (p-IRE), all of which are involved in the cellular unfolded protein response. Prolonged exposure of cells to AgNPs damaged calcium (Ca2+) homeostasis, increased the length of contact sites between the ER and mitochondria, altered IP3R function by the increased levels of phosphatase and tensin homolog deleted on chromosome ten (PTEN) in the ER and enhanced mitochondrial Ca2+ uptake. Finally, Ca2+ overload and disrupted
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