期刊
TOXICOLOGY LETTERS
卷 291, 期 -, 页码 101-111出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2018.02.018
关键词
Furosine; Toxicity; Kidney and liver injury; Cell apoptosis; Inflammatory necrosis response
类别
资金
- Key Laboratory of Quality & Safety Control for Milk and Dairy Products, Ministry of Agriculture
- Laboratory of Quality and Safety Risk Assessment for Dairy Products, Ministry of Agriculture
- Institute of Animal Science, Chinese Academy of Agricultural Sciences, China
- Institute of Animal Sciences, Chinese Academy of Agricultural Sciences [17116]
Aim: Furosine is one of the Maillard reaction products (MRPs) and is found in a variety of heat-processed food. Yet its toxicity is still unclear. The present study was designed to assess furosine toxicity in cell models and in CD-1 mice, respectively. Methods: In vitro, the effects of furosine on the cell viability, cell cycle and apoptosis (Hek293, HepG2, SK-N-SH and Caco2) were detected and evaluated, sensitive cell lines and proper dosage of furosine for further animal experiment were determined, and the mechanisms of toxicity were explored. In vivo, the acute toxicity studieswere performed, organ index, hematology parameters, functions of liver/kidney and pathological changes were detected and the target organs were uncovered. Results: Hek293 cells and HepG2 cells were themost sensitive to furosine with respect to cytotoxicity and apoptosis. Furosine inhibited mice weight gain, and affected the functions of liver and kidney. Conclusions: Furosine posed toxic effects on mice liver and kidney, suggested thatthey were the target organs for furosine toxicity. This study for the first time provides evidence that high dosages of furosine pose adverse biological effects on the health of animals through induction of cell apoptosis and activation of inflammatory necrosis response.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据