In vitro evaluation of the genotoxicity of CeO2 nanoparticles in human peripheral blood lymphocytes using cytokinesis-block micronucleus test, comet assay, and gamma H2AX

Engineered nanoparticles (ENPs) are used in a wide range of applications because of their unique properties. Cerium dioxide nanoparticles (CeO2 NPs) are one of the important ENPs, and they can cause negative health effects, such as genotoxicity, in humans and other living organisms. The aim of this work was to analyze the genotoxic effects of short-term (3-24 h) CeO2 NPs exposure to cultured human blood lymphocytes. Three genotoxicity systems cytokinesis-block micronucleus test, comet assay, and gamma H2AX test were used to show the genotoxic potential of CeO2 NPs (particle size <25 nm, concentrations: 6, 12, and 18 mu g/mL). Hydrogen peroxide was selected as the positive-control genotoxic agent. Our results indicate that CeO2 NPs have genotoxic potential on human peripheral blood lymphocytes cells even at 3-24 h exposure under in vitro conditions.