期刊
THERIOGENOLOGY
卷 115, 期 -, 页码 38-44出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2018.04.019
关键词
Laminarin; Porcine; Oxidative stress; Embryo development
资金
- Korean National Research Foundation [2018005880]
- Next-Generation BioGreen 21 Program [PJ013221012018]
- National Natural Science Foundation of China [31601940]
- Jilin Scientific and Technological Development Program of China [20160520049JH]
- Rural Development Administration (RDA), Republic of Korea [PJ013221012018] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Laminarin (LMA), a beta-glucan mixture with good biocompatibility, improves the growth performance and immune response when used as food additives and nutraceuticals. The aim of the present research was to explore the effects of LMA on porcine early stage embryo development, as well as the underlying mechanisms. The results showed that the developmental competence of porcine early stage embryos was dramatically improved after LMA supplementation during the in vitro culture period. The presence of 20 mu g/mL LMA during the in vitro culture period significantly improved cleavage rate, blastocyst formation rates, hatching rate, and total cell number in the blastocyst compared to that in the control group. Notably, LMA attenuated the intracellular reactive oxygen species generation induced by H2O2. Furthermore, LMA not only increased intracellular glutathione levels, but also ameliorated mitochondrial membrane potential. In addition, the expression of a zygotic genome activation related gene (YAPI), pluripotency-related genes (OCT4, NANOG, and SOX2), and hatching-related genes (COX2, GATA4, and ITGA5) were up-regulated following LMA supplementation during porcine early stage embryo development. These results demonstrate that LMA has beneficial effects on the development of porcine early stage embryos via regulation of oxidative stress. This evidence provides a novel method for embryo development improvement associated with exposure to LMA. (C) 2018 Elsevier Inc. All rights reserved.
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