A boronate-based ratiometric fluorescent probe for fast selective detection of peroxynitrite

Given that peroxynitrite (ONOO-) is profoundly associated with health and diseases, a new fluorescent probe ABT was designed and synthesized for detection of ONOO-. ABT manifested not only ratiometric fluorescence signals simultaneously in response to concentrations of ONOO- (within 10 s), but high selectivity and sensitivity towards ONOO- over other physiological relevant species (detection limit = 26.3 nM). Moreover, ABT worked in a broad pH range with biological relevance. Thus, ABT could be used to quantitative detection of ONOO- concentration and has the potential to efficiently monitor ONOO- in living organisms. (C) 2017 Elsevier Ltd. All rights reserved.