期刊
TALANTA
卷 188, 期 -, 页码 385-392出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.05.069
关键词
Phosphopeptide; Enrichment; Polyimide; Magnetic materials; Metal oxide affinity chromatography
资金
- National Natural Science Foundation of China [21675019, 21727811, 21235001, 21475017]
- Fundamental Research Funds for the Central Universities [N160302001, N150502001]
FexOy and ZrO2 nanoparticles co-doped layered porous polyimide, polyimide-FexOy-ZrO2 is prepared with a one-step strategy, shortly termed as PI-FexOy-ZrO2. The layered and porous structure of the polymer offers a supported platform for metallic oxide anchoring, exhibiting a mesopore size of 3.93 nm and providing a surface area of 198.47 m(2) g(-1). The metallic oxides were uniformly and highly dispersed in the PI-FexOy-ZrO2 nanocomposite with percentages of 15.81 and 20.53 wt% for Fe and Zr, respectively. The magnetic FexOy provides driving force for rapid separation. The high doping of ZrO2 facilitates effective enrichment of phosphopeptides, even at a very low mass ratio of 1:1000 for tryptic digest of phosphopeptides/non-phosphopeptides, e.g., beta-casein/BSA in this particular case. In addition, the PI-FexOy-ZrO2 nanocomposite exhibits better adsorption performance to phosphopeptides with respect to commercial titanium dioxide nanoparticles. The effectiveness of low-abundant phosphopeptides isolation and enrichment from human serum is further identified and demonstrated by means of MALDI-TOF MS and LC-ESI-MS/MS.
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