期刊
TALANTA
卷 184, 期 -, 页码 29-34出版社
ELSEVIER
DOI: 10.1016/j.talanta.2018.02.010
关键词
Metal organic framework; Open-tubular capillary column; Liquid chromatography
资金
- National Natural Science Foundation of China [21475044]
- National Key Scientific Instrument and Equipment Development Project [2012YQ120044]
- National Key Technology RD Program [2015BAK44B00]
An open tubular capillary liquid phase chromatographic column (1 m x 25 mu m i.d. x 375 mu m o.d.) was prepared by incorporating metal organic framework particles modified with vancomycin directly into zwitterionic polymer coating synthesized by the copolymerization of [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide and N,N'-methylenebisacrylamide. The incorporation of IRMOF-3 (isoreticular metal organic framework-3) particles improved selectivity of zwitterionic polymer coating with absolute column efficiency reaching 79900 plates for p-xylene. Besides strong hydrophilic interaction, the separation of neutral, basic, and acidic compounds demonstrates that pi-pi stacking interaction and the coordination effect of unsaturated Zn2+ of MOF also contribute to separation of various analytes. The RSD values (run-to-run, day-to-day, column-to-column, n = 3) of retention time of neutral compounds were less than 0.71%, 0.69% and 3.08% respectively, suggesting good repeatability. In addition, the column was applied to the analysis of the trypsin digest of bovine serum albumin, revealing the potential in separating biological samples.
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