期刊
TALANTA
卷 176, 期 -, 页码 40-46出版社
ELSEVIER
DOI: 10.1016/j.talanta.2017.08.007
关键词
Alpha fetoprotein; ICP-MS; Au NPs; Tyramide signal amplification; Human serum
资金
- National Nature Science Foundation of China [21575107, 21375097, 21205090, 21075095]
- National Basic Research Program of China (973 Program) [2013CB933900]
- Large-scale Instrument and Equipment Sharing Foundation of Wuhan University [LF20170799]
In this work, we developed an immunoassay based on tyramide signal amplification (TSA) and gold nanoparticles (Au NPs) labeling for highly sensitive detection of alpha fetoprotein (AFP) by inductively coupled plasma mass spectrometry (ICP-MS). AFP was captured by anti-AFP1 coating on the 96-well plate and labeled by anti-AFP2-horseradish peroxidase (HRP), in which the HRP can catalyze the deposition of biotinylated tyramine on the nearby protein. Then the streptavidin (SA)-Au NPs was labeled on the deposited biotinylated tyramine as the intensive signal probe for ICP-MS measurement. Under the optimal experimental conditions, the limit of detection of the developed method for AFP was 1.85 pg/mL and the linear range was 0.005-2 ng/mL. The relative standard deviation for seven replicate detections of 0.01 ng/mL AFP was 5.2%. The proposed method was successfully applied to the detection of AFP in human serum with good recoveries. This strategy is highly sensitive and easy to operate, and can be extended to the sensitive detection of other biomolecules in human serum.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据