期刊
TALANTA
卷 182, 期 -, 页码 164-170出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.01.055
关键词
MALDI-MS; STEC; Mass spectrometry; Spectral processing; Escherichia coli; Shigatoxigenic E. coli
资金
- Scottish Government Rural and Environment Science and Analytical Services Division (RESAS)
Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.
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