期刊
TALANTA
卷 176, 期 -, 页码 499-508出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.08.062
关键词
Hydrophilic interaction chromatography; Retention mechanism; Stoichiometric displacement; Partition mechanism; Adsorption mechanism
资金
- NSFC of China [21545007]
- Shaanxi Provincial Science and Technology Coordinating Innovation Projects [2013SZS18-K01]
- Foundation of Key Laboratory in Shaanxi Province [2010JS103, 11JS097, 15J8115]
A stoichiometric displacement model for retention (SDM-R) of small solutes and proteins based on hydrophilic interaction chromatography (HILIC) was presented. A linear equation that related the logarithm of the capacity factor of the solute to the logarithm of the concentration of water in the mobile phase was derived. The stoichiometric displacement parameters, Z (the number of water molecules required to displace a solute from ligands) and lgI (containing a number of constants that relate to the affinity of solute to the ligands) could be obtained from the slope and the intercept of the linear plots of lgk' vs. lg[H2O]. The retention behaviors and retention mechanism of 15 kinds of small solutes and 6 kinds of proteins on 5 kinds HILIC columns with different ligands were investigated with SDM-R in typical range of water concentration in mobile phase. A good linear relationship between lgk' and lg[H2O] demonstrated that the most rational retention mechanism of solute in HILIC was a stoichiometric displacement process between solute and solvent molecules with water as displacing agents, which was not only valid for small solutes, but also could be used to explain the retention mechanism of biopolymers in HILIC. Comparing with the partition and adsorption models in HILIC, SDM-R was superior to them.
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