Rapid detection of four mycotoxins in corn using a microfluidics and microarray-based immunoassay system

Mycotoxins threaten human health seriously because they usually exist in food, fodder and commodities. In this study, a rapid and sensitive immunoassay system for commonly encountered mycotoxins was established based on microfluidics and protein microarrays. Four mycotoxins (T-2 toxin, aflatoxin B1, ochratoxin A, and zearalenone) can be automatically detected in a custom-made microdevice within 30 min under the assistance of a prototype of the instrument with a fluid control system and an imaging system. Once the microdevices are fabricated, they are small-sized and user-friendly. Standard curves for each of the studied mycotoxins were generated with a good logistic correlation (R-2 > 0.98). Working ranges from 0.1 to 20 ng/ml were employed in the immunoassay being the limit of detection achieved between 0.03 and 1.24 ng/ml. These values were calculated when the four mycotoxins were present in samples at the same time. Samples of spiked water and field corn were tested to assess the performance of our microfluidic-based detection technique for the mycotoxins. Recovery rates of mycotoxins from spiked water and corn samples were accessed and the results ranged from 80% to 110%, where the infra-assay coefficients of variation were under 15%. In summary, the system can realize rapid and reliable detection of multiple contaminants in actual samples automatically.