Parendozoicomonas haliclonae gen. nov sp nov isolated from a marine sponge of the genus Haliclona and description of the family Endozoicomonadaceae fam. nov comprising the genera Endozoicomonas, Parendozoicomonas, and Kistimonas

Two Gram-stain-negative, facultative anaerobic, motile, rod-shaped strains, S-B4-1U(T) and JOB-63a, forming small whitish transparent colonies on marine agar, were isolated from a sponge of the genus Haliclona. The strains shared 99.7% 16S rRNA gene sequence identity and a DNA-DNA hybridization value of 100%, but were differentiated by genomic fingerprinting using rep-PCRs. 16S rRNA gene sequence phylogeny placed the strains as a sister branch to the monophyletic genus Endozoicomonas (Oceanospirillales; Gammaproteobacteria) with 92.3-94.3% 16S rRNA gene sequence similarity to Endozoicomonas spp., 91.9 and 92.1% to Candidatus Endonucleobacter bathymodiolin, and 91.9 to 92.1% to the type strains of Kistimonas spp. Core genome based phylogeny of strain S-B4-1U(T) confirmed the phylogenetic placement. Major fatty acids were summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c) and 8 (C-18:1 omega 7c/C-18:1 omega 6c) followed by C-10:0 3-OH, C-16:0, and C-18:0. The G + C content was 50.1-51.4 mol%. The peptidoglycan diamino acid of strain S-B4-1U(T) was meso-diaminopimelic acid, the predominant polyamine spermidine, the major respiratory quinone ubiquinone Q-9; phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine were major polar lipids. Based on the clear phylogenetic distinction, the genus Parendozoicomonas gen. nov. is proposed, with Parendozoicomonas haliclonae sp. nov. as type species and strain S-B4-1U(T) (= CCM 8713(T) = DSM 103671(T) = LMG 29769(T)) as type strain and JOB-63a as a second strain of the species. Based on the 16S rRNA gene sequence phylogeny of the Oceanospirillales within the Gammaproteobacteria, the Endozoicomonaceae fam. nov. is proposed including the genera Endozoicomonas, Parendozoicomonas, and Kistimonas as well as the Candidatus genus Endonucleobacter. (C) 2017 Elsevier GmbH. All rights reserved.