Establishment and characterization of a human embryonic stem cell line, NERCe002-A-3, with inducible 14-3-3ζ overexpression

NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3 zeta after exposure to doxycycline. 14-3-3 zeta protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3 zeta protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a >27-fold increase in relative expression of 14-3-3 zeta as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo. (c) 2018 The Authors. Published by Elsevier B.V.