期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 205, 期 -, 页码 40-47出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2018.07.013
关键词
Sweetener; Serum albumin; Binding mechanism; Conformational structure; Activity
类别
资金
- National Natural Science Foundation of China [21571154]
- Natural Science Foundation of Jiangsu Province [BK20161315, BK20151296]
- Natural Science Foundation of Education Department of Jiangsu Province [17KJA610006]
- Jiangsu Fundament of Qinglan Project
- 333 Project
- Six Talent Peaks Project in Jiangsu Province
In this work, the interactions between bovine serum albumin (BSA) and five sweeteners including aspartame (APM), acesulfame (AK), sucralose (TGS), sodium cyclamate (SC), and rebaudioside-A (REB-A) have been studied by multispectroscopic techniques, and molecular simulation in order to provide much useful information for the application of new and safer artificial sweeteners. Fluorescence quenching assays indicated that the formation of complexes between sweeteners and BSA mainly induced the fluorescence quenching of protein and the binding site number were about 1 indicting that there is one mainly binding site of APM, AK, TGS, SC, or REB-A in domain of BSA with relatively weak interactions. Molecular modeling results indicated that hydrogen bonding interactions were the mainly binding forces of sweeteners with BSA. Circular dichroism spectra indicated that APM and REB-A obviously induced the secondary structure changes of BSA. The presence of APM increased the fraction of alpha-Helix of BSA from 65.4% to 73.8%, while the presence of REB-A resulted in decreasing the fraction of alpha-helix of BSA from 65.4% to 512%. The melting temperature studies showed that these five sweeteners except REB-A act as stabilizers to increase the thermal stability of BSA during the thermal denaturation process. In addition, AK, TGS, and SC obviously increased the esterase-like activity of BSA, and such loss of activity of BSA induced by APM and REB-A. (C) 2018 Elsevier B.V. All rights reserved.
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