SIMULTANEOUS ESTIMATION OF GALLIC ACID, ELLAGIC ACID, AND ASCORBIC ACID IN EMBLICA OFFICINALIS AND IN UNANI POLYHERBAL FORMULATIONS BY VALIDATED HPLC METHOD

A simple, rapid, and economic simultaneous HPLC method was developed and validated for the quantification of Gallic acid (GA), Ellagic acid (EA), and Ascorbic acid (AA), in Emblica officinalis Linn. (aamla) and in two poly herbal Unani formulations, containing aamla as an ingredient. Separation was achieved on a reverse phase C18 (250 x 4.6 mm, 5 mu m) column with a mobile phase of 0.1% orthophosphoric acid and acetonitrile in gradient elution. The flow rate was kept as 1 mL min(-1) and detection was carried out at 254 nm. The proposed method was validated as per the ICH guidelines for accuracy, precision, robustness, LOD, and LOQ. The proposed method was linear over a wide range of concentration with a good regression coefficient of more than 0.99. The LOD of method was 0.035 mu g mL(-1), 0.31 mu g mL(-1), and 0.38 mu g mL(-1), whereas LOQ was 0.1 mu g mL(-1), 1.0 mu g mL(-1) and 1.3 mu g mL(-1) for ascorbic acid, ellagic acid, and gallic acid, respectively. The results demonstrated that the proposed method is a simple, reproducible, accurate, economic, and suitable method for the quality control of aamla, its formulations, as well as a large population of plants with anti-oxidant property.