期刊
SOUTH AFRICAN JOURNAL OF BOTANY
卷 114, 期 -, 页码 150-162出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.sajb.2017.11.003
关键词
Cratoxylum formosum ssp pruniflorum; Apoptosis; FTIR; Death receptor pathway; Intrinsic pathway; Topoisomerase inhibitor
资金
- Research Fund for Supporting Lecturers to Admit High Potential Students to Study and Research in her Expert Program, Khon Kaen University
- Erasmus Mundus project TECHNO I - 3rd cohort
Six species of Cratoxylum are found in Thailand. Whether the Cratoxylum formosum ssp. pruniflorum(CFP) has anticancer properties requires investigation. CFP exhibited cytotoxicity against hepatocellular carcinoma HepG2 cells. Based on FTIR microspectroscopy, CFP raised the respective lipid and nucleic acid content and beta-pleated sheet in HepG2 cells, suggesting a change in the secondary protein structure. CFP induced apoptosis by increasing the activities of various caspases. Overexpression of TRAILR2 indicated the extrinsic pathway while expression of Bax/Bcl-2 ratio indicated the intrinsic pathway and decreased expression of Bid indicated cross-talk between the two. CFP alkylated the DNA and caused DNA damage, which may be the initial step in the intrinsic pathway. CFP indirectly and directly inhibited Top IB enzyme activity and decreased PARP-the protein-related DNA repair process. CFP could, thus, protect the resistance mechanism of cancer by reversing the effect of Top as confirmed by apoptosis induction in the resistant HepG2 cells. The phytochemical analysis suggested that the compounds playing an important role in the anticancer activity of CFP are a group of xanthones. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.
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