期刊
SMALL
卷 14, 期 32, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201801131
关键词
acoustofluidics; cancer phenotyping; circulating tumor cells; high-throughput separation; microfluidics
类别
资金
- National Institutes of Health [R01 HD086325, R01 GM112048, R33 EB019785, DCI P30 CA014236]
- National Science Foundation [IIP-1534645]
- Duke Cancer Institute
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [R01HD086325] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [P30CA014236] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R33EB019785] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM112048] Funding Source: NIH RePORTER
The study of circulating tumor cells (CTCs) offers pathways to develop new diagnostic and prognostic biomarkers that benefit cancer treatments. In order to fully exploit and interpret the information provided by CTCs, the development of a platform is reported that integrates acoustics and microfluidics to isolate rare CTCs from peripheral blood in high throughput while preserving their structural, biological, and functional integrity. Cancer cells are first isolated from leukocytes with a throughput of 7.5 mL h(-1), achieving a recovery rate of at least 86% while maintaining the cells' ability to proliferate. High-throughput acoustic separation enables statistical analysis of isolated CTCs from prostate cancer patients to be performed to determine their size distribution and phenotypic heterogeneity for a range of biomarkers, including the visualization of CTCs with a loss of expression for the prostate specific membrane antigen. The method also enables the isolation of even rarer, but clinically important, CTC clusters.
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