期刊
SMALL
卷 14, 期 27, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201801170
关键词
coacervate; human mesenchymal stem cells; inorganic polyphosphate; metabolic energy; nanoparticles
类别
资金
- ERC Advanced Investigator Grant [268476]
- ERC-PoC [324564, 662486, 767234]
- Federal Ministry for Economic Affairs and Energy [ZIM - ZF4294001 CS6),]
- BMBF Grant: NanoOsMed [01DH17034A]
- International Human Frontier Science Program
- BiomaTiCS research initiative of the University Medical Center, Mainz
- Max Planck Society through the Max Planck Partner Group on Colloidal Methods for Multifunctional Materials (CM3) at the University of Valencia
- Spanish Ministry of Economy, Industry, and Competitiveness through a Ramon y Cajal grant [RYC-2013-13451]
Inorganic polyphosphate [polyP] has proven to be a promising physiological biopolymer for potential use in regenerative medicine because of its morphogenetic activity and function as an extracellular energy-donating system. Amorphous Ca2+-polyP nanoparticles [Ca-polyP-NPs] are characterized by a high zeta potential with -34 mV (at pH 7.4). This should contribute to the stability of suspensions of the spherical nanoparticles (radius 94 nm), but make them less biocompatible. The zeta potential decreases to near zero after exposure of the Ca-polyP-NPs to protein/peptide-containing serum or medium plus serum. Electron microscopy analysis reveals that the particles rapidly change into a coacervate phase. Those mats are amorphous, but less stable than the likewise amorphous Ca-polyP-NPs and are morphogenetically active. Mesenchymal stem cells grown onto the polyP coacervate show enhanced growth/proliferation and become embedded in the coacervate. These results suggest that the Ca-polyP coacervate, formed from Ca-polyP-NPs in the presence of protein, can act as an adaptable framework that mimics a niche and provides metabolic energy in bone/cartilage engineering.
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