期刊
SMALL
卷 14, 期 14, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201703857
关键词
click chemistry; Cu detection; graphene
类别
资金
- Ministry of Science and Technology of China [2013YQ190467]
- Chinese Academy of Sciences [XDA09030305]
- National Science Foundation of China [81361140345, 51373043, 21535001, 81701789]
- research and development program of the Beijing Municipal Administration of Hospital [PX2016024]
A fast (1 min), straightforward but efficient, click chemistry-based system that enables the rapid detection of free copper (Cu) ions in either biological fluids or living cells without tedious pretreatment is provided. Cu can quickly induce the conjugation between graphene oxide (GO) and a fluorescent dye via click reaction. On the basis of the high specificity of bioorthogonal reaction and the effective quenching ability of GO, the assay studied in this paper can respond to Cu ions in less than 1 min with excellent selectivity and sensitivity, which is the fastest sensor for Cu as far as it is known. In addition, the application of this system is verified by performing assays in living cells and untreated urine samples from patients suffering from Wilson's Disease. Such a Cu detection system shows great promises in both fundamental research and routine clinical diagnostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据