期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 35, 页码 19709-19717出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b05180
关键词
platinum nanoparticles; heterogeneous catalysts; enzyme mimetics; catechol oxidase-like activity; oxidation of polyphenols
资金
- NSF [DMR-1255377]
- University of Maryland
- Joint Institute for Food Safety and Nutrition, University of Maryland, College Park, MD - FDA [5U01FD001418]
- FDA Nanotechnology CORES Program
Although enzyme-like nanomaterials have been extensively investigated over the past decade, most research has focused on the peroxidaselike, catalase-like, or SOD-like activity of these nanomaterials. Identifying nanomaterials having oiddase-like activities has received less attention. In this study, we demonstrate that platinum nanopartides (Pt NPs) exhibit catechol oiddase-like activity, oxidizing polyphenols into the corresponding o-quinones. Four unique approaches are employed to demonstrate the catechol oxidase-like activity exerted by Pt NPs. First, UV vis spectroscopy is used to monitor the oxidation of polyphenols catalyzed by Pt NPs. Second, the oxidized products of polyphenols are identified by ultrahigh-performance liquid chromatography (UHPLC) separation followed by high-resolution mass spectrometry (HRMS) identification. Third, electron spin resonance (ESR) oximetry techniques are used to confirm the O-2 consumption during the oxidation reaction. Fourth, the intermediate products of semiquinone radicals formed during the oxidation of polyphenols are determined by ESR using spin stabilization. These results indicate Pt NPs possess catechol oxidase-like activity. Because polyphenols and related bioactive substances have been explored as potent antioxidants that could be useful for the prevention of cancer and cardiovascular diseases, and Pt NPs have been widely used in the chemical industry and medical science, it is essential to understand the potential effects of Pt NPs for altering or influencing the antioxidant activity of polyphenols.
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