期刊
SEPARATION AND PURIFICATION TECHNOLOGY
卷 196, 期 -, 页码 183-190出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.seppur.2017.08.041
关键词
Size exclusion chromatography; Retention factor; Ionic liquid-modified silica; Dextran; Polysaccharide
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2014R1A2A2A05002046]
- National Research Foundation of Korea [2014R1A2A2A05002046] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Size exclusion chromatography (SEC) was developed for the separation of large molecules, such as proteins, polymer, peptides, nucleic acid, and polysaccharides. In this study, ionic liquid-modified silica was synthesized to examine the retention behavior of polysaccharides, such as dextran, fucoidan, alginic acid, and laminarin. Commercial SEC column separated large molecules according to their molecular size. However, Ionic liquid modified silica is expected to retention behavior by the functional group between ionic liquid-modified silica surface with polysaccharides. The SilprBimCl column has larger retention than the other ionic liquid-modified silica columns owing to the large pore size and specific physical and chemical properties of the ionic liquids. 2.93 was the largest retention factor in SilprBimCl column from 5 kDa dextran. Under the SilprBimCl column, the molecular weights of fucoidan, alginic acid and laminarin were 205, 245, and 7-650 lcDa respectively.
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