期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 259, 期 -, 页码 797-802出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.12.135
关键词
Fluorescent probe; Peroxynitrite (ONOO-); Far-red fluorescence emission; Borates; Fluorescence bioimaging
资金
- National Natural Science Foundation of China [21777053, 21607053, 21377046]
- Outstanding Young Scientists Award Fund of Shandong Province [BS2013HZ007]
- Postdoctoral Science Foundation of China [2016M592127]
- Shandong Provincial Natural Science Foundation [ZR2017MB014, ZR2014DM011]
- Shandong Provincial Key Research Project [2016GSF117010, 2015GSF117025]
Peroxynitrite (ONOO-) is involved in various physiological and pathological processes. However the detailed mechanisms of ONOO- in the biological systems remain unclear, probably due to the lack of specific and sensitive methods for detecting ONOO- in living cells. Herein, based on the intramolecular charge transfer (ICT) mechanism, we have developed a highly specific and ultrasensitive ratiometric far-red fluorescent probe RFR-PN for monitoring ONOO- in living cells. Experimental results demonstrated probe RFR-PN possesses high specificity toward ONOO- than other various bioactive molecules. Probe RFR-PN could quantitatively and sensitively detect ONOO- with the detection limit of 0.9 nM by the ratiometric fluorescence method. Surprisingly, the fast response of probe RFR-PN for ONOO- (<5 s) was achieved, and it is essential for the real-time detection of ONOO- in the living systems. Moreover, probe RFR-PN was successfully applied to the fluorescence imaging of endogenous ONOO- in living cells. (C) 2017 Elsevier B.V. All rights reserved.
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