期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 255, 期 -, 页码 2495-2503出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.09.054
关键词
Microfluidic immunochip; Graphene oxide; Carbon nanotubes; Salmonella typhimurium; Synergistic effect; Sensitivity
资金
- CSIR, India
- DGIST R&D program of MSIP [17-BT-02]
- Ministry of Science & ICT (MSIT), Republic of Korea [17-BT-02] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A sensitive and selective microfluidic immunochip was fabricated for detection ofSalmonella typhimurium (S. typhimurium) bacterial cells. In this sensor, graphene oxide (GO) nano sheets wrapped carboxylated multiwalled carbon nanotubes (cMWCNTs) composite acted as a transducer material. The colloidal solution of GO-cMWCNTs composite was selectively deposited onto patterned indium tin oxide (ITO) electrode and sealed with polydimethylsiloxane (PDMS) micro channels. The S. typhimurium antibodies (StAb) were in situ biofunctionalized followed by EDC-NHS covalent chemistry via amidation reaction. The presence of abundant functional groups at the GO-cMWCNTs composite improved the loading of antibodies (StAb) against S. typhimurium leading to improved biosensing characteristics. Wrapping of cMWCNTs with GO resulted in superior electron transfer behavior enhancing the sensitivity (162.47 mu A/CFU-1/mLcm(-2)) almost two folds as compared to that based on GO (89.16 mu A/CFU-1/mLcm(-2)) sheets for bacterial cells detection. Besides this, GO wrapped cMWCNTs integrated microfluidics biosensor offered low detection limit as 0.376 CFU/mL and negligible interference due to presence of Escherichia coli (E. coli ( 0157:H7). (C) 2017 Elsevier B.V. All rights reserved.
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