A novel paperfluidic closed bipolar electrode-electrochemiluminescence sensing platform: Potential for multiplex detection at crossing-channel closed bipolar electrodes

In this work, we for the first time reported a paperfluidic crossing-channel closed-BPE based electrochemiluminescence (P-3C-BPE-ECL) platform where multiple band-shaped C-BPEs are situated perpendicular to two parallel channels, and multiplex detection can be achieved in the reporting channel. The paperfluidic devices are easily fabricated by wax and carbon ink-based screen-printing processes. Under optimized conditions, the P-3C-BPE-ECL is applied to the quantitative analysis of hydrogen peroxide (H2O2) and glucose, with corresponding linear ranges of 0.075-10 mM and 0.08-5 mM, and corresponding detection limits of 0.041 mM and 0.03 mM. To our knowledge, this is the first demonstration of paperfluidic C-BPE-ECL method for glucose determination. Based on its acceptable selectivity, the P-3C-BPE-ECL method is used for measurements of glucose in four complex samples (human serum and urine, wine, and glucose injection) and is compared with the traditional methods. The results indicate a good agreement and prove the reliability and accuracy of the proposed platform. Importantly, the proposed method is demonstrated to have the potential for duplex detection of glucose and uric acid, which intensively matches the requirement of those patients simultaneously suffering from diabetes and gout. Therefore, we believe that the P-3C-BPE-ECL could provide a new platform for wide biochemical applications. (C) 2018 Elsevier B.V. All rights reserved.