期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 254, 期 -, 页码 72-80出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.07.057
关键词
Liquid crystal; 4-Cyano-4-pentylbiphenyl; Graphene oxide; Chitosan; Phospholipids; Antibacterial
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2016R1D1A1B03935782]
- Gachon University research fund [GCU-2016-0181]
Understanding the mechanism of the cellular interaction of graphene oxide (GO)-based materials is vital for their biomedical and environmental applications. In this study, we developed a promising method for imaging the interaction between chitosan (CS)-reinforced GO and the phospholipid membrane based on a nematic liquid crystal (LC) sensing platform. Direct observation of the CS-GO-induced rupture of the pre-adsorbed phospholipid monolayer supported by an LC thin film was achieved using a polarizing optical microscope (POM). The disruption process was clearly monitored in real-time using this method. Moreover, because lipids at the Gram-negative bacterial cell membrane could be transferred onto the interfaces of micro-meter-sized sessile LC droplets, we found the LC droplets could be used to distinguish live Escherichia coli (E. coli) cells from dead cells. CS-GO efficiently reduced the cellular integrity of E. coli which was detectable by LC droplets; thus, the LC-based approach could be used for monitoring the antibacterial activities of GO-related materials. These experimental LC sensing platforms offer simple and direct observation methods for evaluating the effect of GO-based materials on the cell membrane and may be useful in corresponding antibacterial applications. (C) 2017 Elsevier B.V. All rights reserved.
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